AgarCultTM
Xylose Lysine Desoxycholate Agar (XLD)
A selective medium for the isolation of salmonellae and shigellae from clinical specimens and foods. By ISO 6579, ISO 19250, ISO 21567
| Formula | g/l |
| Yeast extract | 3 |
| L-Lysine hydrochloride | 5 |
| Xylose | 3.75 |
| Lactose | 7.5 |
| Sucrose | 7.5 |
| Sodium desoxycholate | 1 |
| Sodium chloride | 5 |
| Sodium thiosulphate | 6.8 |
| Ferric ammonium citrate | 0.8 |
| Phenol red | 0.08 |
| Agar | 13.5 |
| Total | 53.93 |
| pH: 7.4 ± 0.2 at 25°C |
Preparation
# Dissolve 54g in 1000ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Heat with frequent stirring until boiling (DO NOT AUTOCLAVE)
# Cool to 45-50°C, mix well and dispense into appropriate sterile containers
Quality Control (35-37°C/18-24 H)
| Organism | Ref. No. | Result |
| Salmonella
Typhimurium |
ATCC 14028 | Good growth; red colonies with black centre |
| Salmonella
Enteritidis |
ATCC 13076 | Good growth; red colonies with black centre |
| Escherichia coli | ATCC 25922 | Inhibited yellow colonies |
| Enterococcus faecalis | ATCC 29212 | No growth |
Storage
Store the dehydrated medium tightly closed in a dry place at room temperature and use before the expiry date on the label.
Dehydrated medium: 2-30°C
Ordering Infomation
| Code | Products | Package |
| XLD500 | AgarCult Xylose Lysine Desoxycholate Agar (XLD) | 500g/Bottle |