AgarCultTM
Violet Red Bile Lactose Agar – VRBL
The medium use the isolation and enumeration of coliforms in foodstuffs.
By ISO 4832, FDA-BAM, APHA
Formula | g/l |
Enzymatic Digest of Animal Tissues | 7 |
Yeast extract | 3 |
Sodium Chloride | 5 |
Bile Salt | 1.5 |
Lactose | 10 |
Neutral red | 0.03 |
Crystal violet | 0.002 |
Agar | 15 |
Total | 41.5 |
pH: 7.2 ± 0.2 at 250C |
Preparation
# Dissolve 41.5g in 1000ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Heat with frequent stirring until boiling (DO NOT AUTOCLAVE)
# Cool to 45-50°C, mix well and dispense into appropriate sterile containers
Quality Control (37°C-24 H)
Organism | Ref. No. | Result |
E.coli | ATCC 25922 | Red/purple colonies |
S.typhmurium | ATCC14028 | Colorless colonies |
E.faecalis | ATCC 19433 | Inhibited |
Storage
Once opened keep powdered medium closed to avoid hydration and use before the expiry date on the label.
Dehydrated medium: 2-30°C
Ordering Infomation
Code | Products | Package |
VRBL500 | AgarCultTM Violet Red Bile Lactose Agar | 500g/Bottle |
VRBL9010 | AgarCultTM Violet Red Bile Lactose Agar (90mm) | 10Plates/Box |