AgarCultTM
Violet Red Bile Glucose Agar – VRBG
The medium use the isolation and enumeration of enterobacteria in foodstuffs. By ISO 21528, EP, USP
| Formula | g/l |
| Enzymatic Digest of Animal Tissues | 7 |
| Yeast extract | 3 |
| Sodium Chloride | 5 |
| Bile Salt | 1.5 |
| Glucose | 10 |
| Neutral red | 0.03 |
| Crystal violet | 0.002 |
| Agar | 15 |
| Total | 41.5 |
| pH: 7.2 ± 0.2 at 250C |
Preparation
# Dissolve 41.5g in 1000ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Heat with frequent stirring until boiling (DO NOT AUTOCLAVE)
# Cool to 45-50°C, mix well and dispense into appropriate sterile containers
Quality Control (37°C-24 H)
| Organism | Ref. No. | Result |
| E.coli | ATCC 25922 | Red/purple colonies |
| S.typhmurium | ATCC14028 | Red/purple colonies |
| E.faecalis | ATCC 19433 | Inhibited |
Storage
Once opened keep powdered medium closed to avoid hydration and use before the expiry date on the label.
Dehydrated medium: 2-30°C
Ordering Infomation
| Code | Products | Package |
| VRBG500 | AgarCultTM Violet Red Bile Glucose Agar | 500g/Bottle |
| VRBG9010 | AgarCultTM Violet Red Bile Glucose Agar (90mm) | 10Plates/Box |

