AgarCultTM
Sheep Blood Agar Base
Base medium for the preparation of blood agar plates
Formula | g/l |
Peptone | 4.5 |
Tryptone | 14 |
Yeast extract | 4.5 |
Sodium chloride | 5 |
Agar | 12 |
Total | 40 |
pH: 7.3 ± 0.2 at 250C |
Preparation
# Dissolve 40g in 1000ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Autoclave at 121°C for 15 minutes
# Cool to 45-50°C, add 50-100ml sheep blood or horse blood
# Mix well and dispense into appropriate sterile containers
Quality Control (37oC – 24H)
Organism | Ref. No. | Result |
Bacteroides fragilis | NCTC 9343 | Growth, grey colonies |
Escherichia coli | NCTC 12241 | Growth, opaque/grey colonies |
Streptococcus pyogenes | NCTC 12696 | Growth, white colonies with β haemolysis |
Storage
Store the dehydrated medium tightly closed in a dry place at room temperature and use before the expiry date on the label.
Dehydrated medium: 2-30°C
Ordering infomation
Code | Products | Package |
SBA500 | AgarCultTM Shepp Blood Agar Base | 500g/Bottle |
SBA5000 | AgarCultTM Sheep Blood Agar Base | 5kg/Bottle |
DSB10 | AgarCultTM Defibrinated Sheep Blood | 10ml/Bottle |
DSB100 | AgarCultTM Defibrinated Sheep Blood | 100ml/Bottle |
DHB10 | AgarCultTM Defibrinated Horse Blood | 10ml/Bottle |
DHB100 | AgarCultTM Defibrinated Horse blood | 100ml/Bottle |
AgarCultTM
Sheep Blood Agar Base
Môi trường cơ bản dùng pha chế thạch máu
Thành phần | g/l |
Peptone | 4.5 |
Tryptone | 14 |
Yeast extract | 4.5 |
Sodium chloride | 5 |
Agar | 12 |
Total | 40 |
pH: 7.3 ± 0.2 at 250C |
Chuẩn bị
# Hòa tan 40g trong 1000ml nước cất (hoặc nước đã lọc)
# Gia nhiệt và khuấy thường xuyên để môi trường hòa tan hoàn toàn
# Khử trùng ở 121° C trong 15 phút
# Làm mát đến 45-50° C, thêm 50-100ml máu cừu hoặc máu ngựa
# Lắc đều và rót vào các dụng cụ chứa vô trùng thích hợp
Quality Control (37oC – 24H)
Organism | Ref. No. | Result |
Bacteroides fragilis | NCTC 9343 | Growth, grey colonies |
Escherichia coli | NCTC 12241 | Growth, opaque/grey colonies |
Streptococcus pyogenes | NCTC 12696 | Growth, white colonies with β haemolysis |
Bảo Quản:
Bảo quản môi trường ở nơi khô ráo, tránh ánh sáng và sử dụng trước hạn dùng.
Nhiệt độ bảo quản: 2-30°C
Thông tin đặt hàng
Code | Products | Package |
SBA500 | AgarCultTM Sheep Blood Agar Base | 500g/Bottle |
SBA5000 | AgarCultTM Sheep Blood Agar Base | 5kg/Bottle |
DSB10 | AgarCultTM Defibrinated Sheep Blood | 10ml/Bottle |
DSB100 | AgarCultTM Defibrinated Sheep Blood | 100ml/Bottle |
DHB10 | AgarCultTM Defibrinated Horse Blood | 10ml/Bottle |
DHB100 | AgarCultTM Defibrinated sheep blood | 100ml/Bottle |