Rimler-Shotts Medium Base (RS Medium) (TDS)

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SPECIFICATION: 500g

RS medium is a selective medium for the isolation of Aeromonas hydrophila from clinical and non-clinical samples

RS medium là môi trường chọn lọc dùng nuôi cấy Aeromonas hydrophila trong mẫu bệnh phẩm và mẫu khác.

TDS-Rimler-Shotts-Medium Base (RS Medium)

AgarCultTM

Rimler-Shotts Medium Base (RS Medium)

RS medium is a selective medium for the isolation of Aeromonas hydrophila from clinical and non-clinical samples

Rimler-Shotts Medium Base

Formula g/l
Yeast extract 3
Maltose 3.5
L-Cysteine hydrochloride 0.3
L-Lysine hydrochloride 5
L-Ornithine hydrochloride 6.5
Sodium thiosulphate 6.8
Ferric ammonium citrate 0.8
Sodium deoxycholate 1
Sodium chloride 5
Bromthymol blue 0.03
Agar 14
Total 46
pH: 7.0 ± 0.2 at 250C

 

Rimler-Shotts Medium Supplements

Formula for 500ml of Medium Vial
Novobiocin 2.5mg

Preparation

# Dissolve 46g in 990ml of distilled water (purified water)

# Heat and frequent stirring to completely dissolve the medium

# Heat with frequent stirring until boiling (DO NOT AUTOCLAVE)

# Cool to 45-50°C, add 2 vials Rimler-Shotts Medium Supplements

# Mix well and dispense into appropriate sterile containers

Quality Control (35-37°C/24H)

Organism Ref. No. Result
Aeromonas hydrophila ATCC 7966 Maltose (+), Yellow; LDC/ODC (-), H2S (-)
Proteus vulgaris ATCC 13315 Maltose (+), Yellow; LDC/ODC (-), H2S (+) Black
Escherichia coli ATCC 25922 Maltose (-); LDC/ODC (+), H2S (-)

 Storage

Once opened keep powdered medium closed to avoid hydration and use before the expiry date on the label.

Dehydrated medium: 2-30°C

Ordering Information

Code Products Package
RSMB500 AgarCultTM  Rimler-Shotts Medium Base 500g/Bottle
RSM10S  AgarCultTM  Rimler-Shotts Medium Supplements 10vials/Box
RSM9010 AgarCultTM  Rimler-Shotts Medium (90mm) 10Plates/Box