AgarCultTM
Plate Count Agar
The medium use for enumeration aerobic micro-organism in water, milk and dairy products by ISO 4833, APHA
| Formula | g/l |
| Enzymatic Digestion of Casein | 5 |
| Yeast Extract | 2.5 |
| Glucose | 1 |
| Agar | 15 |
| Total | 24 |
| pH: 7.0 ± 0.2 at 250C |
Preparation
# Dissolve 24g in 1000ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Autoclave at 121°C for 15 minutes
# Cool to 45-50°C, mix well and dispense into appropriate sterile containers
Quality Control (37oC, 24H)
| Organism | Ref. No. | Result |
| S. aureus | ATCC 6538 | Good growth |
| E. coli | ATCC 8739 | Good growth |
| B. subtilis | ATCC 6633 | Good growth |
Storage
Store the dehydrated medium tightly closed in a dry place at room temperature and use before the expiry date on the label.
Dehydrated medium: 2-30°C
Ordering infomation
| Code | Products | Package |
| PCA500 | AgarCultTM Plate Count Agar | 500g/Bottle |
| PCA5000 | AgarCultTM Plate Count Agar | 5kg/Bottle |
| PCA9010 | AgarCultTM Plate Count Agar (90mm) | 10Plates/Box |

