AgarCultTM
MacConkey Broth
Medium for the detection of E.coli non-sterile products, according harmonized method EP, USP, JP.
| Formula | g/l |
| Peptones | 20 |
| Oxbile | 5 |
| Lactose | 10 |
| Bromocresol purple | 0.01 |
| Total | 35 |
| pH: 7.3 ± 0.2 at 250C |
Preparation
# Dissolve 35g in 1000ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Dispense into test tube
# Autoclave at 121°C for 15 minutes
Quality Control (37°C-24 H)
| Organism | Ref. No. | Result |
| E.coli | ATCC 25922 | Good growth |
Storage
Once opened keep powdered medium closed to avoid hydration and use before the expiry date on the label.
Dehydrated medium: 2-30°C
Ordering Infomation
| Code | Products | Package |
| MCB500 | AgarCultTM MacConkey Broth EP, USP, JP | 500g/Bottle |