AgarCultTM
Listeria Half Fraser Broth Base (Demi Fraser)
Listeria Half Fraser Broth Base is a re-enrichment medium for the isolation of Listeria spp. from food and environmental specimens acc. ISO 11290
AgarCult Listeria Half Fraser Broth Base (Demi Fraser)
| Formula | g/l |
| Enzymatic digest of animal tissues | 5 |
| Enzymatic digest of casein | 5 |
| Beef extract | 5 |
| Yeast extract | 5 |
| Sodium chloride | 20 |
| Disodium hydrogen phosphate anhydrous | 9.6 |
| Potassium dihydrogen phosphate | 1.35 |
| Aesculin | 1 |
| Lithium chloride | 3 |
| Total | 55 |
| pH: 7.2 ± 0.2 at 250C |
AgarCult Listeria Half Fraser Supplement
| Formula for 500ml of medium | Vial |
| Ferric Ammonium Citrate | 0.25g |
| Acriflavin HCL | 6.25mg |
| Nalidixic Acid | 5mg |
Preparation
# Dissolve 27.5g in 500ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Autoclave at 121°C for 15 minutes
# Cool to 45-50°C, add 1 vial of AgarCult Listeria Half Fraser Supplement
# Mix well and dispense into appropriate sterile containers
Quality Control (35°C/26 ± 2 H)
| Organism | Ref. No. | Result |
| Listeria monocytogenes | ATCC 7466 | Growth, black |
| Pseudomonas aeruginosa | ATCC 27853 | No growth |
Storage
Once opened keep powdered medium closed to avoid hydration and use before the expiry date on the label.
Dehydrated medium: 2-30°C
Ready to Use: 2-20°C
AgarCultTM
Listeria Half Fraser Broth Base (Demi Fraser)
Listeria Half Fraser Broth Base là môi trường tiền tăng sinh trong nuôi cấy Listeria spp từ mẫu thực phẩm, mẫu môi trường theo ISO 11290
AgarCult Listeria Half Fraser Broth Base (Demi Fraser)
| Thành phần | g/l |
| Enzymatic digest of animal tissues | 5 |
| Enzymatic digest of casein | 5 |
| Beef extract | 5 |
| Yeast extract | 5 |
| Sodium chloride | 20 |
| Disodium hydrogen phosphate anhydrous | 9.6 |
| Potassium dihydrogen phosphate | 1.35 |
| Aesculin | 1 |
| Lithium chloride | 3 |
| Tổng | 55 |
| pH: 7.2 ± 0.2 at 250C |
AgarCult Listeria Half Fraser Supplement
| Thành phần cho 500ml môi trường | Vial |
| Ferric Ammonium Citrate | 0.25g |
| Acriflavin HCL | 6.25mg |
| Nalidixic Acid | 5mg |
Chuẩn bị
# Hòa tan 27.5g trong 500ml nước cất (hoặc nước đã lọc)
# Gia nhiệt và khuấy thường xuyên để môi trường hòa tan hoàn toàn
# Khử trùng ở 121° C trong 15 phút
# Làm mát đến 45-50° C, bổ sung 1 lọ AgarCult Listeria Half Fraser Supplements.
# Lắc đều và rót vào các dụng cụ chứa vô trùng thích hợp
Đánh giá chất lượng (35°C/26 ± 2 H)
| Chủng vi khuẩn | Mã số | Kết quả |
| Listeria monocytogenes | ATCC 7466 | Mọc tốt |
| Pseudomonas aeruginosa | ATCC 27853 | Không mọc |
Bảo quản
Sản phẩm được đóng kín nắp và đặt nơi khô ráo tránh ánh sang. Sử dụng sản phẩm trước hạn dùng ghi trên bao bì
Bột đông khô: 2-30°C
Sản phẩm sẵn dùng: 2-20°C
Ordering Infomation / Thông tin đặt hàng
| Code | Name | Package |
| CGLIS500 | ChromoGel Listeria Agar Base (ALOA) | 500g/box |
| CGLIS10SS | ChromoGel Listeria Selective Supplement (ALOA) | 10vials/Box |
| CGLIS10SE | ChromoGel Listeria Enrichment Supplement (ALOA) | 10vials/Box |
| CGLIS10SSE | ChromoGel Listeria Selective + Enrichment Supplement (ALOA) (5+5) | 10vials/Box |
| LHFBB500 | AgarCult Listeria Half Fraser Broth Base (Demi Fraser) | 500g/Box |
| LHF10S | AgarCult Listeria Half Fraser Supplement | 10vials/Box |
| LHFBBA500 | AgarCult Listeria Half Fraser Broth Base with Antibiotics (Demi Fraser) | 500g/Box |
| LFAC10S | AgarCult Listeria Ferric Ammonium Citrate Supplement | 10vials/Box |
| LFBB500 | AgarCult Listeria Fraser Broth Base | 500g/Box |
| LF10S | AgarCult Listeria Fraser Supplement | 10vials/Box |
| CGLMA500 | ChromoGel L.mono Agar Base
(Listeria monocytogenes) |
500g/Box |
| CGLM10S | ChromoGel L.mono Supplement
(Listeria monocytogenes) |
10vials/Box |
| LPAB500 | AgarCult Listeria Palcam Agar Base | 500g/Box |
| LP10S | AgarCult Listeria Palcam Supplement | 10vials/Box |
| LOAB500 | AgarCult Listeria Oxford Agar Base | 500g/Box |
| LO10S | AgarCult Listeria Oxford Supplement | 10vials/Box |
| Ready to Use | ||
| RELHFB225S
New |
ReadyEnrichment Listeria Half Fraser Broth (Demi Fraser) 225ml Sterile acc. ISO 11290 | 10bags/Box
100bags/Box 500bags/Box |
| LFBT9 | AgarCult Listeria Fraser Broth
9ml / tube |
50tube/Box |
| CGLIS9010 | ChromoGel Listeria Agar 90mm (ALOA) | 10plates/Box |
| CGLMA9010 | ChromoGel L.mono Agar 90mm
(Listeria monocytogenes) |
10plates/Box |
| LPA9010 | AgarCult Listeria Palcam Agar 90mm | 10plates/Box |
| LOA9010 | AgarCult Listeria Oxford Agar 90mm | 10plates/Box |
| CTRHA50 | ChemTest Rhamnose Rapid Test | 50tests/Box |