AgarCultTM
Edwardsiella Ictaluri Medium
Edwardsiella ictaluri medium is a selective medium for the isolation of Edwardsiella spp.
| Formula | g/l |
| Peptone mix | 20 |
| Phenylalanine | 1.2 |
| Ferric citrate | 1.2 |
| Sodium chloride | 5 |
| Mannitol | 3.5 |
| Bile salts | 1 |
| Colistin | 0.01 |
| Bromthymol blue | 0.03 |
| Agar | 14 |
| Total | 46 |
| pH: 7.1 ± 0.2 at 250C |
Preparation
# Dissolve 46g in 1000ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Autoclave at 121°C for 15 minutes
# Mix well and dispense into appropriate sterile containers
Quality Control (35-37°C/24H)
| Organism | Ref. No. | Result |
| Edwardsiella ictaluri | ATCC 33202 | Growth, green colonies |
| Edwardsiella tarda | NCIMB 2056 | Growth, green colonies |
| Serratia marcescens | ATCC 8100 | Growth, yellow/red colonies |
| Proteus mirabilis | NCTC 10975 | Growth, brown colonies |
Storage
Once opened keep powdered medium closed to avoid hydration and use before the expiry date on the label.
Dehydrated medium: 2-30°C
Ordering Information
| Code | Products | Package |
| EIM500 | AgarCultTM Edwardsiella Ictaluri Medium | 500g/Bottle |
| EIM9010 | AgarCultTM Edwardsiella Ictaluri Medium (90mm) | 10Plates/Box |