DRBC Agar ISO 21527-1 (TDS)

AgarCult^TM

Dichloran Rose-Bengal Chloramphenicol Agar (DRBC)

DRBC Agar is a selective medium for yeasts and moulds associated with food spoilage by ISO 21527-1

Formula	g/l
Enzymatic Digest of Tissue, Plant	5
Glucose	10
Potassium dihydrogen phosphate	1
Magnesium sulphate	0.5
Dichloran	0.002
Rose-Bengal	0.025
Chloramphenicol	0.1
Agar	15
Total	31.627
pH: 5.6 ± 0.2 at 250C

Preparation

# Dissolve 31.6g in 1000ml of distilled water (purified water)

# Heat and frequent stirring to completely dissolve the medium

# Autoclave at 121°C for 15 minutes

# Mix well and dispense into appropriate sterile containers

Quality Control (25°C/3 days)

Organism	Ref. No.	Result
Sacharomyces cerevisiae	ATCC 9763	Good growth; pink coloured colonies
Aspergillus niger	ATCC 9642	White / Yellow mycelium, black spores
Escherichia coli	ATCC 25922	No growth
Bacillus subtilis	ATCC 6633	No growth

 Storage

Store the dehydrated medium tightly closed in a dry place at room temperature and use before the expiry date on the label.

Dehydrated medium: 2-30°C

 Ordering Infomation

Code	Products	Package
DRBC500	AgarCult  Dichloran Rose-Bengal Chloramphenicol Agar (DRBC)	500g/Bottle