AgarCultTM
Dichloran Rose-Bengal Chloramphenicol Agar (DRBC)
DRBC Agar is a selective medium for yeasts and moulds associated with food spoilage by ISO 21527-1
| Formula | g/l |
| Enzymatic Digest of Tissue, Plant | 5 |
| Glucose | 10 |
| Potassium dihydrogen phosphate | 1 |
| Magnesium sulphate | 0.5 |
| Dichloran | 0.002 |
| Rose-Bengal | 0.025 |
| Chloramphenicol | 0.1 |
| Agar | 15 |
| Total | 31.627 |
| pH: 5.6 ± 0.2 at 250C |
Preparation
# Dissolve 31.6g in 1000ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Autoclave at 121°C for 15 minutes
# Mix well and dispense into appropriate sterile containers
Quality Control (25°C/3 days)
| Organism | Ref. No. | Result |
| Sacharomyces cerevisiae | ATCC 9763 | Good growth; pink coloured colonies |
| Aspergillus niger | ATCC 9642 | White / Yellow mycelium, black spores |
| Escherichia coli | ATCC 25922 | No growth |
| Bacillus subtilis | ATCC 6633 | No growth |
Storage
Store the dehydrated medium tightly closed in a dry place at room temperature and use before the expiry date on the label.
Dehydrated medium: 2-30°C
Ordering Infomation
| Code | Products | Package |
| DRBC500 | AgarCult Dichloran Rose-Bengal Chloramphenicol Agar (DRBC) | 500g/Bottle |