ChromoGelTM Salmonella RB Agar (Rambach Agar)
ChromoGel™ Salmonella RB Agar is a selective medium used for the isolation and identification of Salmonella from food and animal feed, water and other materials.
ChromoGelTM Salmonella RB Agar Base
Formula | g/l |
Peptones | 8 |
Sodium Chloride | 5 |
Chromogenic & Selective Mix | 2.7 |
Agar | 17 |
Total: | 32.7 |
pH: 7.3 ± 0.2 at 250C |
ChromoGelTM Salmonella RB Supplement
Formula for 500ml of medium | Vial |
Propylene glycol | 5ml |
Preparation
# Dissolve 32.7g in 1000ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Heat with frequent stirring until boiling (DO NOT AUTOCLAVE)
# Cool to 45-50°C, add 2 vials ChromoGel Salmonella RB Supplements
# Mix well and dispense into appropriate sterile containers
Quality Control acc. to ISO 11133-2
- Productivity control at 37 ± 2ºC / 18-24H
Organism | Inoculated CFU | Result |
Salmonella typhimurium
ATCC 14028 |
103 – 105 | Red to Pink colonies
>30 CFU |
Salmonella Enteritidis
ATCC 13076 |
103 – 105 | Red to Pink colonies
>30 CFU |
Klebsiella pneumoniae ATCC 13883 | 103 – 105 | Blue to violet
>30 CFU |
E.coli
ATCC 25922 |
103 – 105 | Blue to green
>30 CFU |
- Selective control at 37 ± 2ºC / 18-24H
Organism | Inoculated CFU | Result |
Pseudomonas aeruginosa
ATCC 27853 |
106 – 108 | Inhibited |
Staph aureus
ATCC 25923 |
106 – 108 | Inhibited |
Storage
Store the medium tightly closed in a dry place at room temperature and use before the expiry date on the label.
Store temperature: 2-30°C
Ordering Infomation
Code | Products | Package |
CGSALRB500 | ChromoGelTM Salmonella RB Agar Base (Rambach Agar) | 500g/Bottle |
CGSALRB10S | ChromoGelTM Salmonella RB Supplement | 10vials/Box |
CGSALRB9010 | ChromoGelTM Salmonella RB Agar (Rambach Agar) (90mm) | 10Plates/Box |