ChromoGel™ Listeria Agar Base (ALOA) acc ISO 11290 (TDS)

Category:

SPECIFICATION: 500g

$106.56

ChromoGel Listeria Agar completed with selective and enrichment supplements, is a selective and differential medium for the isolation of Listeria spp. from foodstuffs and other samples and for the identification of L. monocytogenes by ISO 11290

ChromoGel Listeria Agar (ALOA) môi trường chọn lọc dùng nuôi cấy định danh listeria monocytogenes trong thực phẩm và môi trường theo ISO 11290

TDS-ChromoGel-Listeria-Agar-ALOA

ChromoGelTM Listeria Agar Base (ALOA)

ChromoGel™ Listeria Agar completed with selective and enrichment supplements, is a selective and differential medium for the isolation of Listeria spp. from foodstuffs and other samples and for the identification of L. monocytogenes by ISO 11290

 Agar Base

Formula g/l
Enzymatic Digest of Animal Tissues 18
Enzymatic Digest of Casein 6
Yeast Extract 10
Sodium pyruvate 2
Glucose 2
Magnesium glycerol phosphate 1
Magnesium sulphate 0.5
Sodium chloride 5
Lithium chloride 10
Disodium hydrogen phosphate 2.5
5-Bromo-4-Chloro-3-Indolylß-D-Glucopyranoside 0.05
Agar 13
Total 70
pH: 7.2 ± 0.2 at 250C  

Selective supplement

Formula for 500ml of medium Vial
Nalidixic Acid 10 mg
Ceftazidime 10 mg
Amphotericin B 5 mg
Polymyxin B 38350 UI

Enrichment supplement

Formula for 500ml of medium Vial
L-α-Phosphatidylinositol 1g

 Preparation

# Dissolve 70g in 1000ml of distilled water (purified water)

# Heat and frequent stirring to completely dissolve the medium

# Autoclave at 121°C for 15 minutes

# Cool to 45-50°C, add 2 vials of ChromoGel Listeria Selective Supplement and 2 vials of ChromoGel Listeria Enrichment Supplement

# Mix well and dispense into appropriate sterile containers

Quality Control (37°C-24 H)

Organism Ref. No. Result
L.monocytogenes ATCC 19111 growth – green-blue colonies surrounded by an opaque halo
L.monocytogenes ATCC 13932 growth, green- blue colonies surrounded by an opaque halo
L.innocua ATCC 33090 growth- green-blue colonies without opaque halo
E.faecalis ATCC 19433 inhibited
C.albicans ATCC 10231 inhibited
E.coli ATCC 25922 inhibited

Storage

Store the dehydrated medium tightly closed in a dry place at room temperature and use before the expiry date on the label.

Dehydrated medium: 2-30°C

Ready to use plates keep at 2-8 ºC

Ordering Infomation

Code Products Package
CGLIS500 ChromoGelTM Listeria Agar Base 500g/Bottle
CGLIS10SS ChromoGelTM Listeria Selective Supplement 10vials/Box
CGLIS10SE ChromoGelTM Listeria Enrichment Supplement 10vials/Box
CGLIS10SSE ChromoGelTM Listeria Selective + Enrichment Supplement (5+5) 10vials/Box
CGLIS9010 ChromoGelTM Listeria Agar (90mm) 10pcs/Box