ChromoGelTM Listeria Agar Base (ALOA)
ChromoGel™ Listeria Agar completed with selective and enrichment supplements, is a selective and differential medium for the isolation of Listeria spp. from foodstuffs and other samples and for the identification of L. monocytogenes by ISO 11290
Agar Base
| Formula | g/l |
| Enzymatic Digest of Animal Tissues | 18 |
| Enzymatic Digest of Casein | 6 |
| Yeast Extract | 10 |
| Sodium pyruvate | 2 |
| Glucose | 2 |
| Magnesium glycerol phosphate | 1 |
| Magnesium sulphate | 0.5 |
| Sodium chloride | 5 |
| Lithium chloride | 10 |
| Disodium hydrogen phosphate | 2.5 |
| 5-Bromo-4-Chloro-3-Indolylß-D-Glucopyranoside | 0.05 |
| Agar | 13 |
| Total | 70 |
| pH: 7.2 ± 0.2 at 250C |
Selective supplement
| Formula for 500ml of medium | Vial |
| Nalidixic Acid | 10 mg |
| Ceftazidime | 10 mg |
| Amphotericin B | 5 mg |
| Polymyxin B | 38350 UI |
Enrichment supplement
| Formula for 500ml of medium | Vial |
| L-α-Phosphatidylinositol | 1g |
Preparation
# Dissolve 70g in 1000ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Autoclave at 121°C for 15 minutes
# Cool to 45-50°C, add 2 vials of ChromoGel Listeria Selective Supplement and 2 vials of ChromoGel Listeria Enrichment Supplement
# Mix well and dispense into appropriate sterile containers
Quality Control (37°C-24 H)
| Organism | Ref. No. | Result |
| L.monocytogenes | ATCC 19111 | growth – green-blue colonies surrounded by an opaque halo |
| L.monocytogenes | ATCC 13932 | growth, green- blue colonies surrounded by an opaque halo |
| L.innocua | ATCC 33090 | growth- green-blue colonies without opaque halo |
| E.faecalis | ATCC 19433 | inhibited |
| C.albicans | ATCC 10231 | inhibited |
| E.coli | ATCC 25922 | inhibited |
Storage
Store the dehydrated medium tightly closed in a dry place at room temperature and use before the expiry date on the label.
Dehydrated medium: 2-30°C
Ready to use plates keep at 2-8 ºC
Ordering Infomation
| Code | Products | Package |
| CGLIS500 | ChromoGelTM Listeria Agar Base | 500g/Bottle |
| CGLIS10SS | ChromoGelTM Listeria Selective Supplement | 10vials/Box |
| CGLIS10SE | ChromoGelTM Listeria Enrichment Supplement | 10vials/Box |
| CGLIS10SSE | ChromoGelTM Listeria Selective + Enrichment Supplement (5+5) | 10vials/Box |
| CGLIS9010 | ChromoGelTM Listeria Agar (90mm) | 10pcs/Box |