AgarCultTM
Campylobacter Blood Free Agar Base
Blood Free Campylobacter Selectivity Agar Base
Campylobacter Blood Free Medium
Modified Charcoal Cefoperazone Desoxycholate Agar (mCCDA)
Charcoal Cefoperazone Desoxycholate Agar (CCDA)
Campylobacter Blood Free Agar Base is a selective medium for the selective isolation of Campylobacter from human, animal, avian and environmental specimens by ISO 10272
Agar Base
| Formula | g/l |
| Peptic digest of animal tissue | 10 |
| Beef Extract | 10 |
| Casein enzymic hydrolysate | 3 |
| Sodium chloride | 5 |
| Charcoal | 4 |
| Sodium Desoxycholate | 1 |
| Ferrous Sulphate | 0.25 |
| Sodium Pyruvate | 0.25 |
| Agar | 15 |
| Total | 48.5 |
| pH: 7.4 ± 0.2 at 250C |
Campylobacter Blood Free Supplement
mCCDA Supplement
CCDA Supplement
Suplement
| Formula for 500ml of medium | mg/vial |
| Cefoperazone | 16 |
| Amphotericin B | 5 |
Preparation
# Dissolve 48.5g in 1000ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Autoclave at 121°C for 15 minutes
# Cool to 45-50°C, add 2 vials of Campylobacter Blood Free Supplement
# Mix well and dispense into appropriate sterile containers
Quality Control (37°C/24-48 H)
| Organism | Ref. No. | Result |
| Campylobacter jejuni | ATCC 29428 | Good growth |
| Escherichia coli | ATCC 25922 | Inhibited |
Storage
Once opened keep powdered medium closed to avoid hydration and use before the expiry date on the label.
Dehydrated medium: 2 – 30°C
Supplement: 2 – 8°C
Ordering Infomation
| Code | Products | Package |
| CBFAB500 | AgarCultTM Campylobacter Blood Free Agar Base (mCCDA/CCDA) | 500g/Bottle |
| CBF10S | AgarCultTM Campylobacter Blood Free Supplement (CCDA/mCCDA) | 10vials/Box |
| CBFA9010 | AgarCultTM Campylobacter Blood Free Agar (mCCDA/CCDA) 90mm | 10plates/Box |