Campylobacter Bolton Broth Base ISO 10272, FDA-BAM(TDS)

AgarCult^TM

Campylobacter Bolton Broth Base

Bolton Broth Base

Campylobacter Bolton Broth Base is a selective enrichment medium for Campylobacter in food and animal feed, environmental samples and other materials.by ISO 10272, FDA-BAM

Agar Base

Formula	g/l
Enzymatic Digest of Animal Tissues	10
Lactalbumin Hydrolysate	5
Yeast extract	5
Sodium chloride	5
α-Ketoglutaric Acid	1
Sodium Pyruvate	0.5
Sodium Disulfite	0.5
Sodium Carbonate	0.6
Haemin	0.01
Total	27.6
pH: 7.4 ± 0.2 at 250C

Campylobacter Bolton Supplement

Bolton Supplement

Suplement

Formula for 500ml of medium	Vial
Vancomycin	10mg
Cefoperazone	10mg
Trimethoprim lactate	10mg
Amphotericin B	5mg

Preparation

# Dissolve 27.6g in 1000ml of distilled water (purified water)

# Heat and frequent stirring to completely dissolve the medium

# Autoclave at 121°C for 15 minutes

# Cool to 45-50°C, add 2 vials of Campylobacter Bolton Supplement and 50ml of Lysed Horse Blood

# Mix well and dispense into appropriate sterile containers

 Quality Control (37°C/24-48 H)

Organism	Ref. No.	Result
Campylobacter jejuni	ATCC 29428	Good growth
Escherichia coli	ATCC 25922	Inhibited

 Storage

Once opened keep powdered medium closed to avoid hydration and use before the expiry date on the label.

Dehydrated medium: 2 – 30°C

Supplement: 2 – 8°C

Ordering Infomation

Code	Products	Package
CBBB500	AgarCultTM Campylobacter Bolton Broth Base	500g/Bottle
CB10S	AgarCultTM Campylobacter Bolton Supplement	10vials/Box
LHB100	AgarCultTM Lysed Horse Blood	100ml/Box