AgarCultTM
Campylobacter Blood Broth Base (Preston)
Campylobacter Blood Broth Base (Preston) is a selective enrichment medium for Campylobacter from clinical and other specimens by ISO 10272
Agar Base
| Formula | g/l |
| Enzymatic digest of animal tissues | 10 |
| Peptone | 10 |
| Sodium chloride | 5 |
| Total | 25 |
| pH: 7.4 ± 0.2 at 250C |
Campylobacter Blood Supplement (Preston)
Preston Supplement
Suplement
| Formula for 500ml of medium | vial |
| Rifampicin | 2.5mg |
| Polymycin B | 1250 IU |
| Trimethoprim | 2.5mg |
| Cycloheximide | 25mg |
Preparation
# Dissolve 25g in 1000ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Autoclave at 121°C for 15 minutes
# Cool to 45-50°C, add 70ml of Lysed Horse Blood and 2 vials of Campylobacter Blood Supplement (Preston)
# Mix well and dispense into appropriate sterile containers
Quality Control (37°C/24-48 H)
| Organism | Ref. No. | Result |
| Campylobacter jejuni | ATCC 29428 | Good growth |
| Escherichia coli | ATCC 25922 | Inhibited |
Storage
Once opened keep powdered medium closed to avoid hydration and use before the expiry date on the label.
Dehydrated medium: 2 – 30°C
Supplement: 2 – 8°C
Ordering Infomation
| Code | Products | Package |
| CBBBPR500 | AgarCultTM Campylobacter Blood Broth Base (Preston) | 500g/Bottle |
| CBPR10S | AgarCultTM Campylobacter Blood Supplement (Preston) | 10vials/Box |
| LHB100 | AgarCultTM Lysed Horse Blood | 100ml/Box |