AgarCultTM
Campylobacter Blood Agar Base (Skirrow)
Campylobacter Blood Agar Base (Skirrow) is a selective medium for the selective isolation of Campylobacter from clinical and other specimens.
Agar Base
| Formula | g/l |
| Pancreatic digest of casein | 13 |
| Heart infusion | 2 |
| Yeast extract | 5 |
| Sodium chloride | 5 |
| Agar | 15 |
| Total | 40 |
| pH: 7.3 ± 0.2 at 250C |
Campylobacter Blood Supplement (Skirrow)
Skirrow Supplement
Suplement
| Formula for 500ml of medium | vial |
| Vancomycin | 5mg |
| Trimethoprim | 2.5mg |
| Polymyxin B | 1250 IU |
Preparation
# Dissolve 40g in 1000ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Autoclave at 121°C for 15 minutes
# Cool to 45-50°C, add 70ml of Lysed Horse Blood and 2 vials of Campylobacter Blood Supplement (Skirrow)
# Mix well and dispense into appropriate sterile containers
Quality Control (37°C/24-48 H)
| Organism | Ref. No. | Result |
| Campylobacter jejuni | ATCC 29428 | Good growth |
| Escherichia coli | ATCC 25922 | Inhibited |
Storage
Once opened keep powdered medium closed to avoid hydration and use before the expiry date on the label.
Dehydrated medium: 2 – 30°C
Supplement: 2 – 8°C
Ordering Infomation
| Code | Products | Package |
| CBABSK500 | AgarCultTM Campylobacter Blood Agar Base (Skirrow) | 500g/Bottle |
| CBSK10S | AgarCultTM Campylobacter Blood Supplement (Skirrow) | 10vials/Box |
| CBASK9010 | AgarCultTM Campylobacter Blood Agar (Skirrow) 90mm | 10plates/Box |