Campylobacter Blood Agar Base (Butzler) (TDS)*

AgarCult^TM

Campylobacter Blood Agar Base (Butzler)

Campylobacter Blood Agar Base (Butzler) is a selective medium for the selective isolation of Campylobacter from clinical and other specimens.

 Agar Base

Formula	g/l
Meat extract	10
Peptone	10
Sodium chloride	5
Agar	15
Total	40
pH: 7.5 ± 0.2 at 250C

Campylobacter Blood Supplement (Butzler)

Butzler Supplement

Suplement

Formula for 500ml of medium	vial
Novobiocin	2.5mg
Bacitracin	12500 IU
Colistin	5000 IU
Cephazolin	7.5mg
Cycloheximide	25mg

Preparation

# Dissolve 40g in 1000ml of distilled water (purified water)

# Heat and frequent stirring to completely dissolve the medium

# Autoclave at 121°C for 15 minutes

# Cool to 45-50°C, add 70ml of Lysed Horse Blood and 2 vials of Campylobacter Blood Supplement (Butzler)

# Mix well and dispense into appropriate sterile containers

 Quality Control (37°C/24-48 H)

Organism	Ref. No.	Result
Campylobacter jejuni	ATCC 29428	Good growth
Escherichia coli	ATCC 25922	Inhibited

 Storage

Once opened keep powdered medium closed to avoid hydration and use before the expiry date on the label.

Dehydrated medium: 2 – 30°C

Supplement: 2 – 8°C

 Ordering Infomation

Code	Products	Package
CBABBU500	AgarCultTM Campylobacter Blood Agar Base (Butzler)	500g/Bottle
CBBU10S	AgarCultTM Campylobacter Blood Supplement (Butzler)	10vials/Box
CBABU9010	AgarCultTM Campylobacter Blood  Agar (Butzler) 90mm	10plates/Box