Blood Agar Base No.2 ISO 11290, ISO 7932 (TDS)

Category:

SPECIFICATION: 500g

$41.31

Blood Agar Base No.2 is the basic medium for the preparation of blood agar plates by ISO 7932 and ISO 11290-1

TDS-Blood-Agar-Base-No2-ISO-7932-ISO-11290

 

AgarCultTM

Blood Agar Base No.2

Base medium for the preparation of blood agar plates by ISO 7932 and ISO 11290-1

Formula g/l
Protease Peptone 15
Liver extract 2.5
Yeast extract 5
Sodium chloride 5
Agar 12
Total 39.5
pH: 7.3 ± 0.2 at 250C

Preparation

# Dissolve 39.5g in 1000ml of distilled water (purified water)

# Heat and frequent stirring to completely dissolve the medium

# Autoclave at 121°C for 15 minutes

# Cool to 45-50°C, add 50-100ml sheep blood or horse blood

# Mix well and dispense into appropriate sterile containers

.Quality Control (37oC, 24H)

Organism Ref. No. Result
Bacteroides fragilis NCTC 9343 Growth, grey colonies
Escherichia coli NCTC 12241 Growth, opaque/grey colonies
Streptococcus pyogenes NCTC 12696 Growth, white colonies with β haemolysis

 Storage

Store the dehydrated medium tightly closed in a dry place at room temperature and use before the expiry date on the label.

Dehydrated medium: 2-30°C

Ordering infomation

Code Products Package
BA2500 AgarCultTM Blood Agar Base No.2 500g/Bottle
BA25000 AgarCultTM Blood Agar Base No.2 5kg/Bottle
DSB10 AgarCultTM Defibrinated Sheep Blood 10ml/Bottle
DSB100 AgarCultTM Defibrinated Sheep Blood 100ml/Bottle
DHB10 AgarCultTM Defibrinated Horse Blood 10ml/Bottle
DHB100 AgarCultTM Defibrinated Horse blood 100ml/Bottle