AgarCultTM
Hektoen Enteric Agar
Hektoen Enteric Agar is a differential, selective medium for the isolation of Shigella and Salmonella species from enteric pathological specimens by ISO 21567
| Formula | g/l |
| Meat Peptone | 12 |
| Yeast extract | 3 |
| Lactose | 12 |
| Sucrose | 12 |
| Salicin | 2 |
| Bile salts No.3 | 9 |
| Sodium chloride | 5 |
| Sodium thiosulphate | 5 |
| Ammonium ferric citrate | 1.5 |
| Acid fuchsin | 0.1 |
| Bromothymol blue | 0.064 |
| Agar | 14 |
| Total | 75.664 |
| pH: 7.5 ± 0.2 at 250C |
Preparation
# Dissolve 76g in 1000ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Heat with frequent stirring until boiling (DO NOT AUTOCLAVE)
# Cool to 45-50°C, mix well and dispense into appropriate sterile containers
Quality Control (37°C/18-24 H)
| Organism | Ref. No. | Result |
| Salmonella typhimurium | ATCC 14028 | Good growth; blue-green coloured colonies with black centres |
| Shigella flexneri | ATCC 12022 | Good growth; green coloured colonies |
| Escherichia coli | ATCC 25922 | Inhibited or no growth |
| Enterococcus faecalis | ATCC 29212 | No growth |
Storage
Store the dehydrated medium tightly closed in a dry place at room temperature and use before the expiry date on the label.
Dehydrated medium: 10-30°C
Ordering Infomation
| Code | Products | Package |
| HEA500 | AgarCultTM Hektoen Enteric Agar | 500g/Bottle |
| HEA9010 | AgarCultTM Hektoen Enteric Agar 90mm | 10Plates/Box |