AgarCultTM
Sabouraud Chloramphenicol Agar
Sabouraud Chloramphenicol Agar is a selective medium used for the cultivation and isolation of fungi from clinical and nonclinical specimens by ISO 16212, EP, USP
| Formula | g/l |
| Peptic digest of animal tissue | 5 |
| Pancreatic digest of casein | 5 |
| Dextrose | 40 |
| Chloramphenicol | 0.05 |
| Agar | 15 |
| Total | 65.05 |
| pH: 5.6 ± 0.2 at 250C |
Preparation
# Dissolve 65.05g in 1000ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Autoclave at 121°C for 15 minutes
# Cool to 45-50°C, Mix well and dispense into appropriate sterile containers
Quality Control (20-25°C/48-72 H)
| Organism | Ref. No. | Result |
| Aspergillus niger | ATCC 16404 | Good growth |
| Candida albicans | ATCC 10231 | Good growth |
| Saccharomyces cerevisiae | ATCC 9763 | Good growth |
| Escherichia coli | ATCC 25922 | Inhibited |
Storage
Once opened keep powdered medium closed to avoid hydration and use before the expiry date on the label.
Dehydrated medium: 10-30°C
Ordering Infomation
| Code | Products | Package |
| SCA500IEU | AgarCultTM Sabouraud Chloramphenicol Agar | 500g/Bottle |