AgarCultTM
Cytophaga Medium
Cytophaga Agar
Flavobacterium Cytophaga Agar
Cytophaga medium is a selective medium for the isolation of Cytophaga and Flavobacterium species
| Formula | g/l |
| Tryptone | 0.5 |
| Yeast extract | 0.5 |
| Beef extract | 0.2 |
| Sodium acetate | 0.2 |
| Agar | 15 |
| Total | 16.4 |
| pH: 7.0 ± 0.2 at 250C |
Note:
To make selective agar, add Neomycin 5mg/L, Polymycin B 200.000UI/L
Cytophaga Supplements
| Formula for 500ml of Medium | Vial |
| Neomycin | 2.5mg |
| Polymycin B | 100.000UI |
Preparation
# Dissolve 16.4g in 1000ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Autoclave at 121°C for 15 minutes
# Cool to 45-50°C (@)
# Mix well and dispense into appropriate sterile containers
(@)To make selective agar, add Neomycin 5mg/L, Polymycin B 200.000UI/L or 2 vials of Cytophaga Supplements
Quality Control (35-37°C/24H)
| Organism | Ref. No. | Result |
| Flavobacterium columnare | ATCC 49512 | Growth,
Golden Colonies |
Storage
Once opened keep powdered medium closed to avoid hydration and use before the expiry date on the label.
Dehydrated medium: 2-30°C
Ordering Information
| Code | Products | Package |
| CYTM500 | AgarCultTM Cytophaga Medium | 500g/Bottle |
| CYT10S | AgarCultTM Cytophaga Supplements | 10vials/Box |
| CYTM9010 | AgarCultTM Cytophaga Medium (90mm) | 10Plates/Box |